world famous doctor

Chapter 305 Overcoming Medical Issues
Vice President He Yu looked at Zhou Chen proudly, and said in a teaching tone:
"Young man, I have been studying this topic for several years. During the preliminary screening, I have already discovered some genes with significant functional performance, so for the sake of fairness, we should change to a new topic, which is only fair to you... ..."

Having said that, Zhou Chen shook his head, smiled at him modestly and said:

"No need, just study this topic."

Vice President He didn't speak for a while.

He curled his lips and stared at Zhou Chen with eyes full of provocation, disdain and contempt.

He Yu studied this subject several years before Zhou Chen did, but Zhou Chen only started working on it today.

In He Yu's view, not to mention Zhou Chen, a young man who has only learned a little bit of medical science, even if he were an old professor of his own age, he would not be able to surpass himself in research on this subject.

In this regard, Vice President He is still very confident.

But this is better, when the time comes, the subject will be ahead of Zhou Chen, and the position of the deputy dean will still be his own.

After a while, Vice President He said quietly again:

"If you need to know anything about this topic, or if you need any materials and methods, I can teach you, so as not to say that my teacher bullies the students."

Zhou Chen shook his head, and replied in a faint voice:

"Don't worry about it, you can go to your own business, let's do our own things, and try to finish this project as soon as possible."

Hearing this, an unnatural expression flashed across Vice President He's face.

He didn't say anything more, just squinted at Zhou Chen quietly.

Right now, Zhou Chen's every move, every word and deed seems to be provoking the majesty of Vice President He Yu, the old professor.

After finishing speaking, Zhou Chen started to work on his own.

He did not visit the various experiments that Vice President He had done before.

He didn't want to know either.

With a well-known medical system as the backer, are you afraid that there will be problems that cannot be overcome?

Zhou Chen still had this confidence.

Immediately afterwards, Zhou Chen began to obtain materials and specific methods for screening nervous system gene functions from the famous doctor system.

Materials need adult rat brain cDNA library, antisense nucleic acid synthesized by SH Sangon, imported Hamilton microsyringe, domestic stainless steel metabolic cage, water bath, step-down tester, etc.

The experimental animals were inbred BALB/c mice, male (20-22g), clean grade, purchased from SH Experimental Animal Center of ZG Academy of Sciences.

Antisense nucleic acid design and synthesis:

Using bioinformatics methods, select a nucleotide sequence with a length of 20 in the translation initiation region or other regions of the cDNA sequence of the selected gene, design and synthesize antisense nucleic acid.

The design of the random scrambled nucleic acid (scramble) is determined after the sequence is searched for no homologous sequence in the nucleic acid database.

Antisense nucleic acid animals were injected with 10-18 mice for each antisense nucleic acid in vivo, and 10-20 mice were used for the normal saline control group and the random sequence nucleic acid control group respectively.

The antisense nucleic acids of different genes were dissolved in normal saline to a final concentration of 0.5ug/pl, and quantitatively injected into the lateral ventricle of the anesthetized mice (4pl each time, once every 1-2 days, a total of 1 injections), and Set normal saline group and random sequence nucleic acid group as controls.

After the completion of each group of behavioral experiments, blue water was injected at the injection site of the antisense nucleic acid, and a brain slice examination was performed to confirm the accuracy of the injection site.

Next, is the specific mouse behavior experiment.

……

It only took Zhou Chen a few days to catch up with Vice President He.

This is the result of several years of research by Vice President He Yu.

What's more, Zhou Chen's research is more detailed than his, and the data is more accurate.

There are high and low abilities between people.

Although Zhou Chen is young, his medical skills and abilities are much higher than Vice President He's.

Using the speed and rigor of his research on this subject, as well as various correct judgments and experimental data in the research, it can intuitively show Zhou Chen's medical level and technology.

Even though Vice President He's professional title is much higher than Zhou Chen's.

Three days later, Zhou Chen presented the data and results of the experiment in the form of documents and submitted them to the dean.

The dean immediately called several senior MD supervisors in the hospital to check the data.

Based on the adult rat brain cDNA library, a cDNA sub-library of rat brain low-abundance expressed genes was established by differential screening method.

Combining random sequencing and bioinformatics analysis, 20 genes (gene numbers 1 to 20) expressed in Daying brain were selected from the above-mentioned low-abundance expressed gene sublibrary, including:
Different types of genes such as transcriptional regulators, signal transduction molecules, axon growth and neuron activity-related proteins, etc., designed and synthesized specific antisense nucleic acids for these genes, and used them in mouse behavior experiments.

There was no significant difference between the random sequence nucleic acid group and the normal saline group in each behavioral model, and they were the same control group as the normal saline group. A total of 20 experimental mice were used for the 266 kinds of antisense nucleic acids and the two control groups.

神经系统功能基因小鼠行为学检测平台的应用初筛实验结果经t检验后显示：在20种反义核酸中有14种与对照组在不同的行为学模型上存在不同程度的差异（P<0.05，P<0.01，P<0.001)，其中差异非常显著的（P<0.001)有5种……

Obtaining these information can provide an overall functional information platform for in-depth study of specific gene functions, and promote the in-depth development of gene function research.

At the same time, the establishment of the animal behavior detection platform also provides a technical method system for the phenotypic research of transgenic animals and gene knockout animals, which has puzzled researchers at home and abroad for many years.

Experimental zoology is an important branch of zoology. If it can play a role in the study of gene function, it will become a bridge connecting zoology with genetics and neurobiology.

This kind of intersection takes animal behavior as the main research method, which will add new connotations to the ancient research method of animal behavior, and a large amount of modern content in the research method, which will not only provide new information for the functional research of new genes in the nervous system. It has opened a new starting point for in-depth development, and it is undoubtedly of great significance to the further development of zoology.

These senior doctoral supervisors and professors were unanimously satisfied with Zhou Chen's experimental results.

And what is even more shocking is that, faced with such a complex and profound research topic, Zhou Chen actually obtained the result and overcame the problem in only three days.

In the dean's office, everyone cast incredible glances at Zhou Chen.

In any case, they couldn't believe that the person who conquered such a medical subject so quickly was only a young man in his early 20s.

He's just Gui Peisheng who just arrived at the hospital not long ago?

This……

This is impossible.

Among the people present, Vice President He Yu, who was competing with Zhou Chen for the subject, looked cold. He squinted his eyes and stared at Zhou Chen closely. He didn't know what he was thinking at the moment.

(End of this chapter)