I cultivate mushrooms in warhammer

【Day 1】

【You used Chaos Pact Yeast and Primordial Crystal Fungus as experimental subjects.】

【You created 100 portions of fungal suspensions containing both types of fungi and prepared culture plates using the dilution plating method.】

【95 samples showed severe mutations, specifically manifesting as contamination by other bacteria and deep signs of Chaos corruption. Please see the observation images from the holographic microscope. At this time, you decide?】

A holographic image appeared before his eyes, the contents of which could only be described as appalling.

Perfectly fine colonies had transformed into nightmarish shapes, sprouting fleshy limbs or growing long, black-haired tendrils.

These experimental samples were definitely unusable.

Li Nuo silently recited in his mind:

“I decide to discard the mutated samples and render them harmless.”

【95 mutated samples have been rendered harmless. You continue to cultivate the remaining 5 samples.】

【Day 16 to Day 30】

【The remaining 5 samples have mutated. At this time, you decide?】

“I decide to discard them and abandon this simulation.”

Li Nuo wasn't surprised by this result; it was just his first attempt, and failure was normal.

He paced around his private laboratory, deeply recalling the microbiology knowledge he had learned.

Gradually, more knowledge points about “antagonism” surfaced in his mind.

Li Nuo slowly nodded, realizing where his problem lay.

For the past half-year, he had spent most of his time conducting gene-related simulations, and the experimental procedures in the field of microbiology had become unfamiliar, to the point where he had overlooked several key areas.

After clarifying his experimental ideas, Li Nuo sat down at the table and started a new simulation.

This time, he chose manual control instead of automatic, personally manipulating each experimental step, just like when he had first transmigrated.

“System, start the antagonism experiment.”

【Please set the experimental subjects.】

“Target strain: Chaos Pact Yeast. Antagonistic strain: Primordial Crystal Fungus.”

【Please set the culture medium.】

Li Nuo narrowed his eyes and pondered for a moment:

“Use PDA culture medium to cultivate Primordial Crystal Fungus, and LB culture medium to cultivate Chaos Pact Yeast. The raw material for the LB culture medium is blue-purple Ghost Mushroom.”

The main components of PDA culture medium are potato, glucose, and agar.

When Li Nuo first transmigrated, he used synthetic starch and orange sugar to replace potato and glucose.

Now that he had money, he bought genuine potatoes and glucose from the agricultural world, so the agar culture medium he was using at this time was a true PDA culture medium.

LB culture medium, also known as lysogeny broth, mainly consists of tryptone, yeast extract, and sodium chloride, which can promote the growth of other bacteria and further expand bacterial contamination.

Chaos corruption is not bacterial contamination.

When expanding Chaos corruption, materials related to negative emotions should be selected, rather than traditional LB culture medium raw materials.

Therefore, Li Nuo specifically had the system use blue-purple Ghost Mushrooms to make LB culture medium.

This Ghost Mushroom has very strong negative emotions, and emotions are Chaos's favorite “dessert,” which should further promote Chaos corruption.

【Culture medium settings completed.】

【Please set the instruments and environmental parameters.】

Li Nuo took a deep breath and recited the content he had prepared in advance:

“Use a sterile operating table, constant temperature incubator, centrifuge, chromatograph, holographic microscope, and single-molecule analyzer as experimental instruments. Refer to conventional experimental protocols for environmental parameters.”

【Instrument and environmental parameter settings completed.】

【Please set the reagents.】

Reagents are used to test whether antagonism has an impact on Chaos corruption.

A material that is very sensitive to Chaos corruption must be used as a reagent.

So, what meets the requirements?

Li Nuo first thought of Purity Seals.

This item is a must-have tool for Space Marines in the 40k era, with a milky white parchment and a red wax seal.

When encountering Chaos corruption, the parchment will burn, alerting the Space Marines with flames and smoke.

But now it's the 30k era, and the Emperor hasn't started selling hooks on the Golden Throne yet, so where can he find Purity Seals?

Li Nuo crossed his arms, frowned, and searched through the fungal warehouse.

After looking around, Li Nuo's eyes lit up slightly.

He realized he had entered a dead end.

In fact, as long as the life structure of a certain fungus is fragile enough, it can be used as a reagent.

Sufficiently fragile means easy to change.

Easy to change means easy to corrupt.

Assuming the amount of Chaos contamination required to corrupt other substances is 1, then the amount of contamination required to corrupt this fungus may only be 0.01.

Once he understood this point, he had many fungi to choose from.

“System, use blastospores of Saccharomyces cerevisiae and sterile water to make the reagent.”

The cell wall of blastospores is very thin, and the protective structure is very simple, making it easily affected by the external environment and Chaos.

【Reagent settings completed.】

【Start simulation.】

【Day 1, you inoculate Primordial Crystal Fungus in the center of the PDA plate, with a fungal cake diameter of 5mm. You inoculate Chaos Pact Yeast around the Primordial Crystal Fungus, 3cm away from it, forming an inhibition zone.】

【Room temperature 28 degrees Celsius, relative air humidity 80%, no light, sterile environment.】

【Day 5, you measure the radius of the inhibition zone and calculate the inhibition rate, resulting in 13%, indicating that Primordial Crystal Fungus has an inhibitory effect on Chaos Pact Yeast.】

【Day 10, you calculate the inhibition rate again, resulting in 26%, indicating that the inhibitory effect increases over time.】

【Day 15, the fungal cake comes into contact with the inhibition zone, and the Primordial Crystal Fungus mutates. At this time, you decide?】

Hmm, not bad.

Li Nuo crossed his arms, nodded in satisfaction, and thought to himself that it was indeed so.

Primordial Crystal Fungus cannot directly resist Chaos Pact Yeast. After the two come into contact, the probability of Primordial Crystal Fungus being corrupted is 100%.

This point is sufficiently proven by the two previous experiments.

So why does Primordial Crystal Fungus have an inhibitory effect on Chaos Pact Yeast?

According to Li Nuo's experience, this is due to metabolites.

The next step is to conduct antagonism activity analysis of the metabolites.

“System, start a new simulation experiment. All experimental parameters remain unchanged, but this time, first obtain the extract of Primordial Crystal Fungus metabolites.”

【Day 1 to Day 7】

【You allow Primordial Crystal Fungus to ferment at a room temperature of 28 degrees Celsius, filter its fermentation broth using a 0.22 μm thick filter membrane, and obtain sterile fermentation products.】

【You extract the fermentation products using ethyl acetate and concentrate them by rotary evaporation to obtain the fermentation product extract.】

【At this time, you decide?】

“Use the filter paper disc diffusion method to determine the inhibition rate of the extract.”

【Day 7, you use Chaos Pact Yeast and sterile water to prepare a fungal suspension and spread it evenly on the LB plate.】

【You apply the extract to the filter paper disc, attach it to the LB plate, and incubate at a room temperature of 37 degrees Celsius for 24 hours.】

【Day 8, you measure the inhibition zone, and the inhibition rate is 55%, indicating a significant inhibitory effect.】

Good, very good, the metabolites of Primordial Crystal Fungus do have an inhibitory effect.

This makes things easier.

“Separate the different components in the metabolites and test the inhibitory effect of each separately. Select the metabolite with the most significant inhibitory effect and use a mass spectrometer to obtain its molecular formula.”

【Day 9 to Day 15】

【Through numerous experiments, you determine that the inhibitory effect comes from an unknown metabolic component. You use a mass spectrometer to obtain its molecular formula, and the results are as follows…】

A string of molecular formulas appeared in his field of vision, extremely complex and dazzling.

Li Nuo aimed at it and threw out an analysis fungus to obtain relevant information.

【Name: Metabolite that can inhibit Chaos corruption.】

【Analysis results:】

【The inhibition rate of this metabolite on Chaos Pact Yeast reaches 99%.】

【The metabolic mechanism for generating this metabolite is determined by the gene fragment of Primordial Crystal Fungus: Frag015754.】

With the gene fragment number, gene editing can be performed.

Li Nuo started the fungal gene simulation.

“System, use Primordial Crystal Fungus as the experimental subject, scan its gene sample, and find the gene fragment numbered Frag015754.”

【Scanning completed.】

【Gene fragment: Frag015754.】

【Function: This fragment is related to some metabolic functions of Primordial Crystal Fungus.】

【When Primordial Crystal Fungus absorbs subspace energy with emotions, it secretes a specific metabolite.】

【Affected by this fragment, the secretion efficiency of the metabolite is 1%. The secretion efficiency can be improved through gene editing.】

Li Nuo took a slight breath, a smile rising on the corner of his mouth:

“Pause the current fungal gene simulation and start automated fungal gene simulation to find the gene code to improve secretion efficiency.”

【You have paused the “Bone Drinker RNA Vaccine R\u0026D Project”.】

【Project progress has been saved.】

【Start new automated simulation.】

【Day 1】

【…】

Once automatic simulation is involved, time slips away like flowing water.

In the blink of an eye, 26 days and nights passed in busy work.

This evening, as Li Nuo was about to enjoy dinner, the automatic simulation yielded results.

【Day 62407713】

【You are making the 1622600538th attempt.】

The newest novels are first published on Liu 9 Shu Ba!

【You are using the Empire Medical Department's universal CRISPR-Cas9 design tool.】

【You extract a DNA sequence containing 91 nucleotides from the Archeocrystal Fungus, whose 52' end is complementary to the experimental subject, and whose 21' end binds to the Cas9 protein, forming a new gene fragment.】

【You knock out the corresponding gene fragment of the experimental subject and replace it with the new gene fragment.】

【Confirmed by fungal simulation experiments, the efficiency of the Archeocrystal Fungus in secreting specific metabolites has increased dramatically to 100%, and the secretion of other metabolites has stopped.】

"Hiss—increased to 100% all at once?! Then is this fungus still an Archeocrystal Fungus?"

Li Nuo entered the private laboratory, sat down at the table, stared at the colony on the holographic microscope image, and activated Analyze Fungus.

The analysis results almost made him jump to the ceiling!

【Name: Archeocrystal Fungus (70% Atavism)】

【Type: Ancient Saint Creation】

【Analysis Results:】

【The degree of atavism has increased by 10%, and the amount of warp energy single crystals produced per Terra hour has increased from 1 kilogram to 5 kilograms.】

【In addition, this fungus can be used to remove Chaos corruption. However, please note that the process of removing Chaos corruption can only be performed in the system's fungus simulation function, and cannot be practically operated in the real universe.】

My heavens!

This is too unexpected!

It's simply a double blessing!

"Emperor, I wronged you. If you didn't sell your 'groove' to the Chaos demons, I wouldn't have obtained the Chaos Pact Yeast, wouldn't have thought about purifying it, and wouldn't have been able to promote further atavism of the Archeocrystal Fungus through gene editing."

"Sorry, Emperor. Your 'groove' is a good thing, sell more, the more the better. This way I can take back more good things belonging to the Ancient Saints from Chaos. Wahaha~"

Li Nuo was so excited that his body trembled, and he immediately started the experiment to remove Chaos corruption.

10 minutes later, the system text refreshed.

【You have removed the Chaos corruption from the Chaos Pact Yeast and completed the purification work.】

【The Chaos Pact Yeast has become Seal Yeast, which can seal warp entities in the real universe through spiritual fermentation.】

【The life structure of this yeast is complex, but its gene expression ability is outstanding, and it can be used as an expression vector for gene editing.】

【Affected by gene purity, the spiritual fermentation effect and the expression effect of gene editing are unstable. Improving gene purity can improve this.】

【Current gene purity is unknown, unlock after obtaining the gene template.】

【When the gene purity reaches 90%, this fungus is domesticated.】

Okay, family members, the Chaos Pact Yeast has transformed into Seal Yeast, and the new gene expression vector is done.

Improving gene purity or something, can be put on hold for now, not in a hurry.

"I remember in the fungus warehouse, there is also a kind of fungus corrupted by Chaos..."

Li Nuo rummaged through the fungus warehouse and clicked on the panel of the Warp Mold.

Its panel introduction is like this:

【Name: Unknown Name Pathogenic Strain】

【Analysis Results:】

【This Warp Mold was transformed by Nurgle, and its toxicity is only effective on a very small number of plants and is harmless to humans.】

【The biggest feature of this thing is the 'Warp Superposition State'. It can be observed by the material universe, but it cannot be captured by the material universe.】

【When you try to inoculate and cultivate it, it will immediately return to the warp, to prevent you from conducting in-depth research on it.】

This Warp Mold comes from diseased White Ridge Tobacco.

Its fungal ability is very powerful, and it once made Li Nuo's heart burn.

【Fungal Ability: Warp Walk】

【Ability Description:】

【You fade out of the material universe and enter the surface layer of the warp. In the material universe, you are in an invisible state and a wall-walking state, with no physical collision volume, and can ignore obstacles and are immune to any damage from the material universe. However, you will emit a spatial signal from the warp, which will be detected by people and objects with psychic identification capabilities.】

【Unlock Requirement: 100 skill points.】

【After successful domestication, the unlock requirement is reduced to 1 skill point.】

【The domestication process will not arouse Nurgle's vigilance.】

【Please do not forcibly unlock and use this fungal ability before the domestication is completed! Otherwise, terrible results will occur!】

Li Nuo couldn't domesticate the Warp Mold before, but now it's different.

Archeocrystal Fungus, activate!

10 minutes later.

【You have successfully removed the Chaos corruption on the Warp Mold and completed the domestication work.】

【After domestication, the Warp Mold becomes Ghost Mold.】

【The panel of this mold is as follows:】

【Name: Ghost Mold】

【Analysis Results:】

【An ancient mold passed down from the Dark Age of Technology.】

【Its mycotoxins can be used to prepare 'Invisibility Potion', granting the drinker temporary 'Warp Walk' ability.】

【Fungal Ability: Warp Walk.】

【Unlock Requirement: 1 skill point.】

Li Nuo unlocked the fungal ability he had been coveting for a long time without saying a word, quickly cultivated 100 tons, and then activated Warp Walk.

The private laboratory faded in color in his vision, becoming a grayscale photo, the buzzing of the experimental instruments disappeared, and the surroundings became extraordinarily quiet.

Li Nuo tried to stretch out his right hand towards the wall, widened his eyes, and watched most of his arm pass through the wall, but he didn't feel the slightest discomfort.

"Awesome, now I have a wall-hacking cheat!"

Then look at the three Black Knights in the corner.

A layer of hazy white light appeared on their surface.

When approaching, Li Nuo felt an obvious sense of frustration, and the white light was pushing him away forcefully.

This is the psychic shield inherent in the warp single crystal, which only acts on warp creatures and warp energy, giving the Black Knights unparalleled spatial resistance.

Warp Walk is achieved through the warp, and is also within the scope of the shield's effect.

Li Nuo stretched out his arm to touch the white light, and found that this thing looked like a light curtain, but it had the touch of steel, and it was truly indestructible.

Li Nuo nodded slightly, extremely satisfied with the single crystal's warp resistance.

He ended Warp Walk and turned his head to look at the psychic reading table on the wall.

A sharp curve protruded upwards, and the reading level at the highest point reached an astonishing Gamma level!

Warp Walk is actually a psychic ability that only Gamma-level psychics can master, it's truly terrifying!

The walls of this laboratory are fitted with anti-psychic lead plates, which can prevent the psychic fluctuations on both sides from spreading to the other side, so there is no need to worry about the psychic little experiment just now being noticed by people and instruments outside.

Li Nuo calmed his mind, suppressed the agitation in his heart, and operated on the system interface.

Fungal gene simulation and fungal cultivation simulation can be carried out simultaneously.

Cultivation simulation is used to produce single crystals to replenish the inventory of commonly used fungi.

As for gene simulation, Li Nuo considered it this way.

First, develop an RNA vaccine against the Bonedrinkers, and then use Seal Yeast as an expression vector to produce Bonedrinker spores with a gene purity of over 90%, unlocking more fungal abilities.

The RNA vaccine mixes a portion of the fungal phage virus RNA, and also contains the ferment of reverse parasitic yeast, as well as a self-developed nucleotide repair solution.

Its kill rate is 100%, its safety is far superior to the previous generation of vaccines, and only one vaccination is required to be immune to Bonedrinker parasitism for life.

The self-developed nucleotide repair solution is cheap, with the cost of each branch being less than 1 Throne Coin, but the repair effect on the gene chain is limited to mutations caused by fungal infections.

After the RNA vaccine and the Bonedrinker fungal ability are unlocked, then use gene simulation to promote further atavism of the Archeocrystal Fungus.

The two fungal simulations are advancing side by side, and a bright future is in sight.

After dinner, after seeing off Ti Duo, who came to freeload, Li Nuo began his daily work as the Green-skin Pal Master.

The save summary reminded him that it was time to log on to the Blood Raven Boy's account and deal with those 13 Dark Angel Inner Circle veterans.

【Save 8: Blood Raven Boss - Kroen.】

【Save Summary:】

【After a 26-day warp voyage, the escort ship is about to reach its destination.】

【The anesthetic you infused into the Inner Circle veterans has tempered their will and drug resistance.】

【Now they have almost adapted to the presence of anesthetic ingredients and are largely immune to such ingredients.】

【Log in before the Inner Circle veterans go berserk! Lest the Blood Raven Boys die!】