Siheyuan: Returning from studying abroad, with extraordinary understanding
Chapter 320 Successfully Produced Penicillin
It would be a waste to just leave the Penicillium collected in the morning.
Might as well use waste.
"That's great!"
"Changqing, is that enough time?"
Qian Yuanqing was a little worried.
"There's not enough time. Changqing, please come back tomorrow! It would be better if Changqing could stay a few more days!"
"Yes, yes! This way I can learn more knowledge."
Everyone hoped that Lin Changqing would stay a little longer.
However, Lin Changqing dashed their hopes:
"A few hours is enough. You have to believe me."
"Ah?" Everyone was dumbfounded!
So fast!
They all hoped to go slower, but they had to believe in Lin Changqing's ability.
If I say a few hours, it means a few hours.
Ignoring everyone's frustrated mood, Lin Changqing continued:
"After collecting the Penicillium that secretes penicillin, the next steps are filtering, extracting, decolorizing, adjusting the pH, crystallizing, separating and drying. It doesn't take long."
"However, the Penicillium we collected contained a lot of impurities, so the penicillin we finally obtained might not be of high purity."
"But don't worry, I have a way to purify it!"
After hearing Lin Changqing's words, everyone was happy but also a little disappointed.
I am happy that I can see how the legendary penicillin is made in the afternoon. What is sad is that after making penicillin, Lin Changqing will leave.
"Changqing, we really don't want to let you go. It would be great if you could stay!"
Qian Yuanqing looked at Lin Changqing with a pleading tone.
"Yes, Changqing, as long as you are here, I estimate that it won't be long before the medical technology of our Dragon Country will catch up with Eagle."
Lin Changqing's performance surprised Zhou Yuan. Even his mentor in Eagle Sauce did not have such wild and brilliant ideas as Lin Changqing.
No matter what difficulties he encounters, Lin Changqing can always come up with effective solutions.
Zhou Yuan had seen this in the morning. Even though he studied abroad in Eagle Sauce and graduated with a doctorate, when he encountered the difficulties this morning, he thought for a long time and came up with some useless solutions.
Even if we think of a solution, none of it is applicable, either because of a lack of equipment or medicine.
Obviously, in the country, the Eagle Sauce approach is simply not applicable.
But Lin Changqing was able to come up with a brilliant solution, and it was simple and practical!
He was ashamed of himself.
"I probably won't stay, but if you have any questions in the future, you can come to me."
Medicine is very important, but it cannot make the Dragon Kingdom strong and prevent the Dragon Kingdom from being bullied.
Only technology can make the Dragon Kingdom strong.
Naturally, he had to contribute his own strength to the industrialization of Longguo.
After dinner, no one asked for a rest. Instead, they rushed back to the laboratory, wanting to see the birth of penicillin as soon as possible.
Lin Changqing did not refuse and returned to the laboratory with everyone.
In the laboratory.
Lin Changqing assigned work to everyone and divided them into groups to prepare test tubes, flasks, pH solutions and other things.
"Everything is ready, Changqing, let's get started."
"it is good."
Lin Changqing was already prepared and came to the laboratory table.
Pick up the large beaker of Penicillium and show it to everyone.
"These are all Penicillium that can secrete penicillin. I've already talked about this this morning, so I won't go into details. I'll start filtering and extracting penicillin now."
In the morning, only 357 grams of Penicillium that can secrete penicillin were collected from two trucks of rotten fruits and food residues.
Lin Changqing picked out the Penicillium that produced a high amount of penicillin and cultured it in a culture medium, leaving only 312 grams.
Of course, the cultured Penicillium needs to be selected.
It cannot be guaranteed that all the Penicillium strains that are selected at one time are high-yielding penicillin-producing Penicillium.
It would take multiple selections, and Lin Changqing had already taught them how to select, so he didn't have to worry about it anymore.
Lin Changqing followed the experimental process step by step. He did not waste any time and explained while he was operating.
Everyone observed carefully and kept it firmly in mind.
Just like that, after several filtration and extraction steps, it is time to reach the decolorization step.
"The next step is decolorization. For this step, we choose to add activated carbon. Using the adsorption effect of activated carbon, impurities such as pigments and heat sources in the solution are adsorbed on its surface."
As Lin Changqing spoke, he added activated carbon and then stirred it thoroughly.
The amount of activated carbon added needs to be calculated based on actual conditions, neither too much nor too little.
If there is too much, penicillin will be easily absorbed. If there is too little, the decolorization will not be clean and harmful substances will remain.
Lin Changqing introduced all of these in the document, and also indicated the ratio between penicillin and activated carbon.
The proportion is the best he could simulate, neither too much nor too little, just right.
After adding activated carbon and stirring evenly, let it sit for a while.
Lin Changqing was also not idle, answering various questions for everyone.
In everyone's mind, Lin Changqing has become a medical treasure trove, and a medical treasure trove that can answer any questions.
Just a few words of advice can make them suddenly realize.
What Lin Changqing didn't know was that Zhao Yiming and others had already regarded him as the God of Medicine.
A few years later, when Zhao Yiming and others were interviewed, the most common words they said were:
"He is the god of medicine, omniscient and omnipotent. What I learned in the laboratory that day was more than what I had learned in the first half of my life."
Of course, these are all things that happened later. Lin Changqing has done many great things.
After more than three hours, we finally reached the crystallization step.
If there weren't so many impurities, Lin Changqing would not have spent so much time going back and forth for the purification process several times.
You know, when several types of penicillin are mixed together, some of them have no anti-disease effect.
In order to obtain more pure penicillin, Lin Changqing purified it several more times.
"Okay, now we have a pure penicillin solution. Now we come to the most critical step - crystallization."
"It is important to adjust the pH of the solution first, and then add the precipitant. We use the simplest method, direct crystallization, and add sodium acetate."
There was no sodium acetate in the laboratory, but this did not pose a problem for Lin Changqing. Before the experiment, he asked people to prepare the reagents and carried out two experiments at the same time.
Sodium acetate has already been produced.
Sodium acetate is an organic compound, white crystal, easily soluble in water, and has a wide range of uses, including as a buffer, catalyst, precipitant, etc.
There are other methods for penicillin crystallization, such as azeotropic crystallization, but the process is a bit more complicated.
The direct crystallization method is relatively simple and easy to operate.
Lin Changqing added a certain amount of sodium acetate, stirred it evenly and waited for penicillin crystals to appear.
"Everyone come and have a look, penicillin crystals will appear soon."
Everyone moved closer to the flask and observed it carefully.
After a while, white crystals appeared in the flask.
"Look, the crystals appear!"
boom!
Everyone is excited!
They saw penicillin crystals with their own eyes!
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