Rebirth of the God-level Xueba
Chapter 1193: 1 pot stir
"The step of crystallization is the most important. You must pay attention and then pay attention, keep the process in mind, and recite the steps silently in your heart..."
"This step of dissolving is the most important, absolutely pay attention and pay attention to see if there is any precipitation? Don't waste the raw materials. Also, always remember that after a dissolution, no more solvents are allowed, so be sure to calculate and use it. the amount."
"Don't give me the calculations here. Experiments, you only need to do the calculations!"
Tao Xuelin whirled around in the ion channel laboratory and gave orders.
This is what he is very good at. Before he became a full professor, Tao Xuelin's main job was to lead the people under his hands. Of course, before the 1980s, the most work was actually the cooperation of various units. When the superior leaders had any ideas, whether it was the leader of Peking University, the leader of the Ministry of Education, the leader of the Ministry of Culture, the leader of the Academy of Sciences, or even the leader of Beijing , Anything that is somewhat related can form a research team.
Ten years earlier, the research team’s projects were diverse, especially for the teachers of the biology department. It’s simpler, how to improve the efficiency of manure, and how to design a brand new one if it’s more complicated. Manure, it's a bit more difficult, what you eat can produce better manure...
At that time, it was not a single school or a certain research institute that worked independently. Although many projects can be done by only one or two people, in the eyes of the leader, how can one or two people appear to pay attention to the project? , How can I replace the project I finally came up with?
The signature of Peking University cannot be ignored. Therefore, for a long time, Tao Xuelin has done "big projects".
Tao Xuelin was frustrated for a period of time. In fact, for a period of time, many academic elites were frustrated. They always felt that they were wasting time and talent...
However, experience is never wasted.
Although Tao Xuelin has done many useless large projects, the experience of large projects has been preserved after all.
In the ion channel laboratory, he also quickly found his place, which is to manage the production of bovine rhodopsin protein.
How to produce is Su Xiankai's job. In the early days, he tried various methods while doing production.
With the improvement of the experiment progress, the nature of Su Xiankai's work has actually slowly changed.
Just like Yang Rui did the production method of coenzyme Q10 back then, the research that Su Xiankai did was actually slowly biased towards the research of production method. Moreover, when the scope of his research became wider and wider, bovine rhodopsin protein In fact, it has become the same as Coenzyme Q10, which can be divided into labor division and cooperation and enter a state of professional production.
In the 1980s, biopharmaceuticals were actually a laboratory production model, including the WestJet factory established by Yang Rui. A total of 10 or 20 people, 20 or 30 people, can form the production department of the factory. Everyone operates the equipment. It's similar to the enlarged version of the laboratory, some do not even need to enlarge, just use the laboratory for production.
Extracting coenzyme Q10 from bovine heart, extracting insulin from bovine pancreas, and extracting bovine rhodopsin protein from bovine eye are all a kind of model.
Yang Rui and Su Xiankai helped them a little and passed all the skills they currently master to Tao Xuelin.
Tao Xuelin only took a few days to increase the production capacity of bovine rhodopsin protein several times.
This is not an initial increase of several times, but an increase after Su Xiankai has been intensively cultivated for a long time.
The addition of Tao Xuelin also allowed the production of bovine rhodopsin protein to not only exceed the 100-fold increase requested by Yang Rui, but also to exceed it.
This is the embodiment of Tao Xuelin's management ability.
The six research dogs were also allocated, and the improvement of Gu Qiang's group was not so obvious.
Although Gu Qiang's experimental ability is strong, his management is not strong. On the contrary, due to Gu Qiang's too strong personality, the graduate students brought by Tao Xuelin still don't like the cooperation with Gu Qiang.
However, no one would dare not listen to Tao Xuelin's orders. Unless anyone does not want to graduate, otherwise, for graduate students, the tutor is the sky, the tutor is the earth, the tutor is the brilliant and great flower, the tutor is you who have tortured to death thousands of times in your dreams, but make you pretend to be in the daytime People who are abused in an enjoyable manner.
Tao Xuelin's mental state is also very good. Putting his laboratory aside and entering Yang Rui's laboratory is also a huge challenge for Tao Xuelin, who has seen him in his 40s. It is naturally very pleasant to be able to locate oneself quickly.
He was pacing in the big laboratory assigned to him, watching the work of the students one by one, more serious than when he was in his own laboratory.
Gu Qiang was a little burnt, and he looked at Tao Xuelin angrily and helplessly, and said: "It used to be Su Xiankai, but now I have added another Tao Xuelin. Now the number of bovine rhodopsin proteome is more than us, so How can I use up more materials."
Tian Bing glanced at him obliquely and said, "I haven't heard of too much material."
"Just ask if you are afraid." Gu Qiang asked while working.
Tian Bing hummed twice, and said, "There is nothing to be afraid of. Director Yang doesn't have any requirements for me."
Inserting soluble protein fragments into G protein coupled receptors is simple to say but not easy to do.
G protein-coupled receptors are still a kind of protein, and the size of the protein is much smaller than that of microorganisms. In other words, you can't see this thing with an ordinary microscope.
It's not easy to insert something into what you can see, and it's too difficult to insert something into something you can't see.
Therefore, it is necessary to verify the stability of the receptor, which is even more difficult.
Tian Bing himself was in pain, but this did not prevent him from taunting Gu Qiang.
Gu Qiang curled his lips and said, "Yang Rui has no quantitative requirements for you, because you haven't achieved any results yet."
Tian Bing smiled: "We just started to do it here, what do you do? If you can't achieve results anymore, let alone Yang Rui, Su Xiankai will have to kill you."
If the bovine rhodopsin protein made by staying up late was wasted, Su Xiankai would indeed beat people angrily.
"It really doesn't work, I just stirred up the bovine rhodopsin protein he sent here." Gu Qiang was fierce. He was really tired recently, especially after Tao Xuelin joined, let alone sleep and eat. It was a trot.
Tian Bing laughed haha and said, "Who wouldn't waste it, be careful that Director Yang hangs you up and beats you."
Hanging up and hitting is a phrase that Yang Rui has often said recently, and everyone else has also learned it.
Gu Qiangqiang argued: "Who said that a pot of stirring is a waste. Anyway, the end of the process is to enter the centrifuge. The preliminary treatment is carried out uniformly, that is, the yield is lower and the efficiency is improved, right? Let's talk about the quantity. After the increase, the pre-processing will be unified, and the overall effect will not be too bad. You haven't seen a few students who do pre-processing and their faces are pale..."
"If I didn't know you were talking nonsense, I would really believe it."
"It can't be said that it's all nonsense..." Gu Qiang said as he spoke, sinking into a state of contemplation.
"What do you think?" Tian Bing had a bad feeling in his heart.
Gu Qiang didn't say a word. After a while, he said, "Actually, think about it carefully. I wasn't **** just now. I said something reasonable."
"What's the point? You're at least wasting 10% of your protein like this."
"Not necessarily. The preprocessing is inherently lossy. The preprocessing is done in batches, and the loss is a bit larger, but we can find a way to optimize it. Compared with before, it is more than 5% at most..."
Gu Qiang thought about the future with a beautiful smile on his face.
Tian Bing reminded: "5% is not too much. Su Xiankai's output has increased to more than 100 times the original. Think about it, if you increase the loss by 5%, it is equivalent to wasting five or six times the previous output."
"When the bovine rhodopsin protein is not enough, 5% must not be wasted. Now the conditions have changed? What is the purpose of Yang Rui to increase our production? Su Xiankai and Tao Xuelin worked hard to make bovine rhodopsin protein. What is it for? Not yet..."
"It's not for you to waste."
"It's for us to have room for choice." Gu Qiang was eloquent, and the more he said it, the more reasonable he felt. After a while, he nodded fiercely and said: "I think we can try."
Tian Bing's eyes jumped desperately and asked, "What do you want to try?"
"Just what you proposed, stir in a pot."
"You brought up the trouble, not me." Tian Bing quickly declared, and tried to dispel Gu Qiang's worries, and said: "Don't be fooling around. Now there are more people. You are like Tao Xuelin. The arrangement is reasonable, even if you work overtime, you will not be able to add a few hours. One-pot stirring will not work, not only the loss of pretreatment, but also the pollution in your later stage. What should you do when it is separated and treated."
"Do whatever you want~www.wuxiaspot.com~ Gu Qiang paused and said: "We did it separately before, just like cooking in the kitchen. You have to prepare each dish one by one. Dishes are prepared in different ways. Doing it together is like a hot pot. Did you eat a lot of Dong Lai Shun’s copper pot last time? Is hot pot worse than stir-fried dishes? "
Tian Bing looked at Gu Qiang like a lunatic, and said, "You are driven crazy by the mission."
"Yeah, I was driven crazy." Gu Qiang lay down with a casual look on your face, clapped his hands, and said, "Anyway, I have to try it once."
"If Yang Rui knew that you were wasting so much bovine rhodopsin protein, he would really hang you up and beat you."
"Hot pot, must the cooking be bad?" Gu Qiang asked again.
Tian Bing said, "You wouldn't just want to explain to Yang Rui like this."
"Talking about hot pot and cooking, Yang Rui should understand it better." Gu Qiang looked ahead and found a perfect reason consciously.
...
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