Rebirth of the God-level Xueba

　　 "Director Yang, where is it inappropriate?" Gu Qiang scratched his head.

　　 "It's because it's too suitable." Yang Rui muttered to herself, "Did you form a complex in the presence of detergent?"

　　 "Yes, the args complex you named, I have tested it, and it can effectively couple in the phospholipid bilayer."

　　 Yang Rui looked up at Gu Qiang, and then asked, "Is there no drop in coupling efficiency? I mean, in the presence of detergent."

In the first phase of the study, detergents were also used to treat g protein coupled receptors, but different from the second phase, in order to ensure the "purity" of the g protein coupled receptors, the second phase always requires The presence of detergents is a higher requirement. The most important point is that once there are detergents, the coupling efficiency of g protein-coupled receptors will be greatly reduced, and it will drop to heinous To the point.

　　 There are two ways to solve this problem. One is to complete a series of experiments before the coupling efficiency drops to an unacceptable level, and the other is to solve the problem of the coupling efficiency decline, or at least extend the high efficiency time.

　　 Yang Rui came to Gu Qiang's experimental record and checked it.

　　 I only saw Gu Qiang’s experimental records. In this part of the record, there is only a short page.

　　 Gu Qiang’s answer is even simpler: "There was a drop in the initial stage. After I changed the solution, the problem of the decrease in coupling efficiency was solved."

　　 Yang Rui's voice was trembling. He pointed to Gu Qiang's experimental record and said, "You changed the lauryl maltoside solution."

　　 "Yes. However, the complex formed in this way was later found to be unstable."

　　 "Yes, it must be unstable."

Gu Qiang nodded and said, "Then I remembered the experiment I did before. I encountered similar situations that were solved by enzymes. I added adenosine triphosphate phosphatase, which happened to be on the complex and gave gdp to the complex. It is hydrolyzed to avoid interaction with gs."

　　 "Yes, it just hydrolyzes." Yang Rui felt speechless.

　　 Gu Qiang said with a guilty conscience: "Is there something wrong? I did it all in accordance with your plan, and nothing was derived from it."

　　 "The problem is that there are no problems anywhere." Yang Rui stared at Gu Qiang for a long time, and said: "Why did you make the plan smoothly?"

　　 "Is Shun Shun Dangdang bad?" Gu Qiang has a somewhat unruly personality, but he has been tamed by Yang Rui a long time ago, but at this time he was a little uneasy.

　　 Yang Rui recalled the development of Gu Qiang’s counter-intuitive "one-pot stir" technology, and his inaccurate experimental skills, and suddenly asked, "Have you brushed your circle of friends?"

　　 Gu Qiang was taken aback for a moment, and said, "No friends are playing tricks on me."

　　 "Okay." Yang Rui sighed and said: "Congratulations, another top-level paper has been made out of your hands."

　　 "Do it?" Gu Qiang was stunned.

　　 "Yes, it's done." Yang Rui confirmed.

　　 "Is it really done?"

　　 "It's really done."

　　 "It's impossible, it's only a few days." Gu Qiang jumped his feet excitedly, his cheeks flushed red.

　　 Yang Rui nodded in agreement, and said, "I don't think it's possible either. It's only a few days."

　　 "Even if the second stage is completed?"

　　 "This is the crystal structure of the complex?"

　　 "Is the crystal structure made?"

　　 Affected by Gu Qiang's cheers, a group of researchers gathered around.

　　 Yang Rui is like a tour guide, telling a passionate story plainly, saying: "Although some mathematical calculations and verification are needed, the crystal structure is almost done."

　　 A group of people looked at each other, and finally came down to one question: "How is it possible!"

　　 Yang Rui sighed in sympathy, not knowing how to explain it.

　　 To say that this story is destined to become a strange anecdote, it is also inseparable from Yang Rui. It is he who gave the customs clearance secrets and gave the full set of plans.

　　 Yang Rui did this to increase the speed and complete the second phase of the research as quickly as possible. Given a full set of plans, the researchers will be able to make modifications and tests in a targeted manner.

　　 However, given a full set of plans, some people actually completed the plan in a few days. This is the most incredible place.

　　 Let alone a Nobel Prize-level scientific research experiment, it is a junior high school experiment. The teacher still gives a full set of experimental programs. Can all students make results?

　　 asked a middle school chemistry teacher to answer this question. He can tell a hundred kinds of strange experimental results, none of which are real experimental results.

　　 A slightly more difficult university experiment, how many students can get results? One of the three, even if the students are studying hard.

　　 In a weaker university, it’s common to have an organic chemistry experiment class and a class with no results.

Not to mention the experimental difficulty of the crystal structure of g protein-coupled receptors. Among the several key points, first of all, we must use the correct detergent and control the relatively appropriate concentration-the requirements are relatively broad and easy to achieve. , But this alone cannot be made by just pulling an experimental dog.

　　 Next, at an appropriate concentration, a complex crystal of g protein-coupled receptor is formed.

　　 There are even fewer researchers who can make this step. If this is used as a graduation requirement for university experiment courses, 90 undergraduates will have to cry.

　　 Then, in the presence of detergents, the coupling of the composite crystals is ensured, and

It is a heavy request.

　　 Historically, kbka discovered that lauryl maltoside solution can solve the problem of coupling. There is no record of how long it took him to do this, but with full of malicious guesses, it is trivial to try three or four years. Of course, it was not kbka who tried it himself. He had doctoral students and assistant researchers, but this can only show how many outstanding researchers' scientific research lives were wasted.

　　 After solving the coupling, the remaining steps, including enzymatic hydrolysis, etc., are not particularly time-consuming, but as far as the normal historical process is concerned, a few months are always necessary. When Yang Rui operated the cheat, he also reserved time for two months.

　　 However, all these, all these golden fingers, the brilliance of geniuses, the painstaking efforts of hard-working scholars, are as ethereal as smoke and dust under the fate of shit.

　　Gu Qiang just did it step by step, the correct steps were more than the trial and error steps, and he did it, and it came out...

　　 In Yang Rui's view, it is as if you have installed a very difficult stand-alone game, and found a strategy for the clearance from the Internet, and then... three lives pass the game!

"Let’s take a look again." Yang Rui said, naming the names, and said: "Su Xiankai, Fan Zhenlong, Tian Bing~www.wuxiaspot.com~ That...Professor Man Hai, Professor Tao Xuelin, you also join in, just follow Gu Qiang’s experimental procedures , Do a repeat experiment. Old Xu, you and I will check the experiment records again. By the way, the experiment table is not allowed to move, and the garbage is not allowed to be dumped. The packaging of all used consumables must be recorded."

　　 The researchers who were named all gathered together.

　　 Gu Qiang still didn’t realize his strong fortune today. He laughed and said, “I don’t need so many people. It’s easy for me to do this experiment.”

　　 "Well, Gu Qiang, you will start another set of your own and do a set of repeated experiments." Yang Rui didn't dare to take it lightly. This is another Nobel Prize achievement. It will be done in a few days. Who dares to believe it?

　　 Gu Qiang didn’t care about changing the experiment table, took all the consumables again, did it, and said quietly, “If you say anything else, I’m the least afraid of doing experiments.”

　　 While talking, Gu Qiang also hummed a song.

　　Only Yang Rui stared at the experimental tables in the laboratory as if facing an enemy, and his mind turned.

　　 The schedule on the white wall is as new as ever. Only at the bottom, two participating laboratories have been added, namely the University of Melbourne in Australia and the Winter Institute in the United States. The progress bars are 27 points and 16 points respectively.

　　 Yang Rui glanced at it and stopped looking at it. If the accuracy of the results can be proved by the triple repeated tests, the progress bar of the Ion Channel Laboratory of Peking University will be approved as 100 points.

　　……

　　 (End of this chapter)