Rebirth of the God-level Xueba
Chapter 1436: Enucleated
Wang Liangcai sat on the bed, staring at the envelope he had retrieved from the finance department, and did not want to look away for a long time.
The envelope is very thick, thick enough to doubt life.
JMC's paper bonus will not be available until the paper is officially published, so Wang Liang is still a little disappointed.
However, the bonus of the achievement award is obviously not comparable to the bonus of publishing a paper.
It was also when he saw this envelope that Wang Liang realized that the laboratory values the results.
After all, completing a topic often represents multiple heavyweight papers. As for the papers, if you want a little more, just brush them more. They are all dependent on the results.
"One is Langyuan Xianpa, and the other is Beautiful Jade Flawless. If you say there is no romance, you will meet him again in this life..."
The opening theme of "A Dream of Red Mansions" came from next door, and Wang Liangcai was awakened abruptly.
The freshly released "Dream of Red Mansions" can be regarded as the most attractive TV series of this year. The so-called empty streets of 10,000 people are not an exaggeration at all.
When the opening song of a TV series is played, you can hear the voice calling home in the yard. Adults and children who are crazy about playing can easily be called home. You can’t go home if you don’t. Friends who are playing together must go home. Watching "Dream of Red Mansions", who will play with you?
There is no TV in Wang Liangcai's suite. This is the only regret. Wang Liangcai went to the cafeteria to watch the big TV two days ago, but today he was a little too lazy to run.
He reached out and grabbed the envelope on the bed, poured out the brand-new banknotes inside, and counted it again, then took out most of them and put them back in the envelope, thinking: my wife wanted to change the color TV for a long time, otherwise, this year Just use this money to change it.
Wang Liangcai divided the rest into three more portions, preparing to save one portion for the family to improve the food, another portion to buy good clothes for his wife and children, and finally, to buy some good books for himself.
After the allocation was completed, Wang Liangcai still couldn't believe the four stacks of money in front of him.
He has never had any monthly income and is capable of four things.
Let alone four, it's just a quarter. They are all amazing.
For example, when you buy clothes, you save some money every month. When the season changes, you can only buy clothes once, and you can't buy them for everyone in the family. It must be the state of buying autumn clothes for my wife this year, and there is no money to buy winter clothes.
It’s even more difficult to replace electrical appliances. It is common to save money for one or two years to buy an electrical appliance. During this period, there can be no big projects to spend money on.
Therefore, when you walk into an ordinary person's house, you can count the electrical appliances with one hand.
Wang Liangcai has no foundation at home. He has worked for many years and became a professor, so he bought a black-and-white TV. There are also electric fans, tape recorders, and single-tube washing machines, which can be regarded as catching up with the national average. As for equipment such as refrigerators and air conditioners, Did not enter the discussion category yet.
Wang Liangcai couldn't help but touched the money with his hand, as if afraid it would run away.
Turning his head, Wang Liangcai couldn't help but grinned bitterly. He was a self-proclaimed literati, but he didn't expect that he loved money so much.
"A gentleman loves money, and he takes it in a good way." Wang Liangcai clenched his fist and decided in secret, he put a pile of money into the quilt, and fell asleep in the dark.
Four o'clock in the morning.
After sleeping for 8 hours, Wang Liang got up from the bed, washed himself, and went to the laboratory.
The dormitory was very close to the laboratory. When he reached the door, Wang Liangcai gave a brief explanation, and the guard put him in without any surprise.
Wang Liangcai was walking and rubbing his hands. When he arrived in the laboratory, he felt his fingers were soft. Then he opened the stove that was pressed yesterday, added coal, and then put on the lab coat, opened the instrument cabinet, and turned on the micromanipulator. Microscope, piezoelectric pulse meter, stereo microscope...
Superovulation and egg retrieval can only be regarded as the preparation stage for cloned sheep. Next, the real challenge begins with "de-nuclearization".
Compared with the first two operations, the latter has a stronger sense of "scientific attributes".
At the very least, more instruments are used.
Judging from the prices of various equipment in 1987, there are only a handful of research institutes in China that can undertake this project.
Wang Liangcai inspected the instrument and prepared the denuclear fluid.
There are two types of sucrose-free liquids and sucrose-free liquids. Various strange hormones can be added to them. Every time you mix something, you can write a small essay.
Even changes in concentration can be regarded as a small issue.
But in Yang Rui's genetic engineering laboratory, such a project is naturally inconspicuous.
Wang Liangcai only made the sucrose-free version and the 3% sucrose version according to the traditional model. Immediately afterwards, he took out the petri dish, dripped it with the denucleation operation, added cell relaxant, then covered with paraffin oil, and finally placed the oocytes of the sheep in the droplets.
It was only the small steps at the beginning that made Wang Liang sweat all over, and the cold in the laboratory had long since been felt.
This is also Wang Liangcai at the academician level. If he is replaced by another player, let alone a newcomer, an old professor who is known for his operation, or a new professor who is a fledgling newcomer, may not be able to play with these few steps.
At the very least, you have to fail for a week or two to complete the prescribed action 100%.
Considering that every failure here will waste one or more oocytes, you can imagine the importance of experienced players.
Not to mention, the next steps are just more complicated continuous movements, more testing techniques.
Tong Dizhou was able to get up from the Belgian cattle back then because of his good technique.
As for why his technique is good? There is a story specifically about it: At that time, the Belgian people had begun to commute to get off work on time. The scientific researchers strictly implemented the work requirements, and went home to play after dinner. Tong first soaked in the laboratory personally on Monday until two or three o’clock in the evening until the security guard. Drive talents away.
This training technique was passed down as Tong Di Zhou returned to China.
"Have you seen the capital at four o'clock in the morning?" For genetics scholars, it is really not a problem.
Not so, how can you gain the reputation of hardship on the road of hardship biology.
Wang Liangcai didn't find it hard to do experiments. Compared with metaphysical physics and suicidal chemistry, biology was finally normal. At the very least, he could improve his value through training.
Sheep's egg cells are too expensive, and they can be used as amphibious frog egg cells like that of comrade Zhou.
However, long hours of training and concentration are definitely inevitable.
Not only that, it is difficult for any experimenter to perfectly remember every experimental step, and it is even impossible for any experimenter to be informed of the latest research trends in the scientific research community at any time.
In this case, you also need to keep reading a lot of literature.
Even with the help of the data team, the time saved is still limited.
Wang Liangcai also read the operating instructions first, and then adjusted the lens of the inverted phase-contrast microscope, began to align the oocytes, and gently waved with the enucleating needle to find the correct position.
After rubbing sore hands, Wang Liangcai finally used the nucleus needle to stick to the cell with the help of the fixed needle~www.wuxiaspot.com~, and attracted the zona pellucida to keep the nucleus needle under negative pressure, and then turned on the piezoelectric pulse meter to try Break the zona pellucida with a strong pulse.
The nucleus is very small.
The cell is small enough, but the nucleus is more compact than the cell.
It is obviously not easy to remove the nucleus from the cell.
The blind suction method currently used by Wang Liangcai has a high success rate, but it is easy to **** too much material in the egg.
The latter is an important nutrient component for the egg cell to develop into an embryo. If too much is sucked away, the embryo will fail to develop completely.
Wang Liangcai also knew this drawback, so he thought while experimenting.
The experiment is like this, there is no perfect experimental method, and it is impossible to stop continuing because the method is not perfect.
He will think about new methods, but it is not unacceptable to use stupid methods to make results if there is no moment of insight.
Unless the stupid method consumes too many resources, most of the experiments are completed by numerous stupid methods connected with a few inspirations.
It was getting clearer, and there were more people in the laboratory, Wang Liangcai was still completely forgetful and continued to work.
From four o'clock in the morning to eight o'clock in the morning, although he worked for four hours, as far as the experiment is concerned, he only did it four times.
And it failed four times.
For an experimental biologist who is used to self-abuse thousands of times, four times are just appetizers.
At nine o'clock in the morning, Gu Qiang Shi Ran came to the laboratory.
He arrived late, but he chose to go to the cafeteria for breakfast first, and come back after he was full, but he first saw Wang Liangcai who was working hard.
"It's early." Gu Qiang couldn't help but curl his lips, but the pace under his feet was three minutes faster.
...
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