Rebirth of the God-level Xueba

"So, from the very beginning, Director Yang decided to reproduce this sheep asexually?"

"It looks like it is."

"Then we will also do asexual reproduction experiments in the future?" Su Fan's tone became more and more solemn.

Wang Liangcai nodded solemnly and said, "It looks like it is."

"Are you kidding? It shouldn't be... at least the cloning of sexual reproduction should be successful first, and then the asexual reproduction should be done?" Su Fan's thinking is going to be confused.

Before that, when everyone talked about cloning, they were talking about sexually reproducing cloning, in which the nucleus of the germ cell was put into the enucleated egg cell.

Therefore, cloning is not a new word.

However, even so, cloning is still a big project in genetic biology, such as the famous boy fish, which is typical of this type.

In the development process of cloning, it basically followed the process from amphibians to aquatic products, then from aquatic products to mammals, and then from mammals to economic animals.

Both Su Fan and Wang Liangcai felt that it was time to clone economic animals, and they had the self-confidence and excitement of a terminator.

However, the sudden news of "asexual reproduction" blinded both of them.

This is equivalent to crossing several levels. It seems that children who have just started to play outdoors will go straight to Mount Everest without taking a helicopter.

The most important thing is that biologists cannot prove that asexual reproduction is really feasible without previous similar experiments.

The normal mode, now using an easier organism, first prove that asexual reproduction is feasible, and then become a mammal, is the simplest and most feasible solution.

But from another perspective, what creature is the easier one?

Don't worry about microorganisms, bigger fish and amphibians? Their genders are inherently ambiguous, and it is common in nature that female fish become male.

The so-called, if you want to talk about a biologically stable creature, at least it must be a mammal.

So, will small mammals be easier to clone? For example, the most common mice and rats in the laboratory?

Not really!

Wang Liangcai, who had been experimenting for a period of time, came to this conclusion after his mind turned.

It takes a certain amount of time to carry out enucleation and nucleation. The embryo development time of mice is only two weeks, and that of sheep is six weeks. The latter is easier than the former by many times.

In this regard, all animals commonly used in laboratories are not suitable for cloning. Because one of the reasons why the laboratory chooses them is fast growth and development.

This is a good choice for experiments with adults. However, when the experiment object is embryos, these animals commonly used in the laboratory are precisely not suitable.

Wang Liangcai raised his head and glanced at Yang Rui, and said in a low voice: "Director Yang has chosen sheep as the donors, so he is a one-time idea."

Su Fan is also a shrewd person. After thinking about it, I know why Wang Liangcai said so, and said helplessly: "Director Yang is not a person who likes to do projects step by step."

Recalling the projects that Yang Rui did before, from the potassium ion channel experiment to the G protein-coupled receptor project, none of them were actually done step-by-step.

Su Fan and the others didn't know Yang Rui's true support. Recalling this at this time, they could only comment like this.

The so-called success is always a case of success, Wang Liangcai has no way to criticize, he can only sigh, thinking that young people just can't hold back their temper.

However, this kind of thought can only be reflected in Wang Liangcai's mind. As far as Yang Rui’s current prestige is concerned, he has long been separated from his age. People don’t like to follow the steps, that is, forge ahead...

"What should I do now?" Su Fan is not a particularly creative person, so he should ask Wang Liangcai first.

"I have never cloned asexual reproduction. If it is feasible, the success rate will be extremely low." Wang Liangcai sighed.

The success rate is an eternal pain in the heart of experimental biologists. Many experiments know that they can be done, and then the success rate is horribly low, and finally give up.

Now that you encounter experiments that may not be able to be done, and the success rate is as low as the instructions, the result is very disgusting-you don't know if your failure is a misoperation or you can't do it.

At this time, Wang Liangcai was extremely eager to have a result prompt board, telling himself which way was doomed to fail.

"We have to increase the early success rate. The previous plan has to be overturned and restarted." Wang Liangcai quickly made a decision, and said: "The success rate of de-nucleation must be increased to at least 80%, and then the core will be injected. "

Su Fan was not surprised that Wang Liang just made this plan.

The step-by-step academic school is like this, it is impossible to fight for luck like Gu Qiang.

It is most common to pile up success rates on small, simple and cheap projects, and give it a last try.

No one is rushing to change from a bicycle to a motorcycle. The academic thinking is that it is good to be able to change a good bicycle. Besides, academicians don’t ride a motorcycle, don’t they?

Su Fan didn't mean to discourage him, so he took a learning attitude and said, "If you want to increase the early success rate, do you want to do a comparative test?"

"Yes, for a comparative test, it's best to find a few more people." Wang Liangcai said, looking around the laboratory.

Doing a comparative experiment is actually doing a small experiment.

For example, whether the operating liquid is sucrose or no sucrose, 2% sucrose or 3% sucrose, are all small details. However, the success rate of the experiment is the accumulation of such small details.

Why is the same egg stripping? Some people have a success rate of 70 to 80%, and some have a success rate of only 20 to 30%. In addition to the difference in technique, it depends on who has noticed more details. Who knows the details.

The comparison experiment is to measure small details.

If you change to a local university, you can publish a paper by doing such a comparative experiment.

A large number of Chinese journals and SCI entry-level journals publish papers of similar level. In fact, Yang Rui’s first paper, the UV spectrophotometry of coenzyme Q10, is just such an article, which solves a single problem in a narrow direction in the related field, so it can only be published in a very low-level journal. Information that can be found by scholars with relevant needs.

In computer retrieval, or in other words, when academic search engines have not yet appeared, such data retrieval also requires a lot of manpower and material resources.

Su Fan didn't need Wang Liang to arrange the task, and said first: "I will go to the data team to remind me, and put the direction I am looking for in the early stage?"

"Yes." Wang Liangcai's eyes still wandered through the crowd.

Because of the new construction of Yang Rui's genetic engineering laboratory, Yang Rui's system is different from that of the ion channel laboratory. It adopts the system of a large laboratory, which allows researchers to join and organize research groups freely, so it is more competitive.

However, the establishment of a research team requires funding and time, and of course also requires subjects.

The 600,000 + 30,000 US dollars that Gu Qiang sought from Yang Rui and the timing of the inverted phase-contrast microscope are all resources of the research team.

Although the research group is still restricted and guided by Yang Rui, compared with the old model of ion channel laboratories, the large laboratory system of genetic engineering laboratories is obviously more independent and more adaptable to larger and larger scales. laboratory.

Theoretically, Wang Liangcai, who was seconded, would be able to bring in people as long as he obtained the consent of other researchers. Anyway, it would be fine to include his name in the paper according to his contribution. The main interest of the laboratory also exists here.

"In the old Haidian District Genetic Engineering Laboratory, researcher Sun Zhengchu has the strongest level and good coordination ability." Su Fan introduced Wang Liangcai in a low voice, then turned around and said, "As for the experimental assistant, I think Qin Yue is good. He used to be a veterinarian of China Animal Husbandry, and he has been seconded to the Haidian District Genetic Engineering Laboratory for a long time. As far as our current project level is concerned, it is a matter of one sentence to transfer him."

"This Qin Yue... is just an experimental assistant, is it a bit too troublesome to transfer over?" Wang Liang was a little hesitant.

Su Fan said carelessly: "You have the right not to use it. We are in a national-level project team now. Think about how the previous projects of the same level were done? The number of people gathered across the country is considered to be few. Now there is a middle shepherd, and they can’t wait to send it over immediately.”

Wang Liang was dumb. He had done experiments with peace of mind in the university before, and he had never participated in a project of this scale.

In biology, there are really not many so-called big projects, and they are all related to medicine. Genetic engineering has always been a minority. Wang Liangcai has never tried to slap Fang Qiu.

"There are definitely not many laboratory assistants. If you want to speak, they are even scarcer than researchers." Su Fan paused, and said: "A good laboratory assistant is hard to find. For Qin Yue, he has veterinary experience and knows how to work in the laboratory. In fact, I have also participated in the cattle embryo transfer project, with rich experience and hard work, which is very appropriate."

There are students under Wang Liangcai's hands, but it is not appropriate to pull the National People's Congress over, and the experimental assistant is necessary.

As Su Fan said, a good experiment assistant is still indispensable. Sometimes, an unwashed test tube may cause an experiment to fail, and a good researcher does not necessarily wash the test tube clean.

"Just follow what you said~www.wuxiaspot.com~ seconded Qin Yue." After Wang Liangcai finalized his decision, he was in a good mood. Finally, he clenched his fists like the characters on TV and said, "Let's do a good job. Right."

"Have a big fight." Su Fan laughed.

As the two laughed, the laboratory became noisy.

Wang Liangcai simply stopped and said with a smile: "Just go over and see how other people are doing."

"Okay." Su Fan followed Wang Liangcai through the public area with multiple instruments, and saw Gu Qiang being surrounded by a group of people.

Su Fan frowned instinctively and asked Tian Bing next to him: "What is he doing with moths?"

Tian Bing said with a sigh, "You absolutely can't think of it. From the start of construction until now, Gu Qiang has never failed to show the nucleus and remove the nucleus. He has just taken the nucleus of the breast cells and is now ready to inject the nucleus."

"I haven't failed in one step..." Su Fan actually wanted to say that there was no failure in one step, and there is nothing great, because as long as you fail one step later, you will lose all previous efforts. Therefore, this is their original intention to continuously increase the success rate.

However, when the first half of this sentence came out, Su Fan couldn't say the second half anymore.

In any case, Gu Qiang's current progress is the number one in the laboratory.

"If his nuclear injection is successful, this will be the first case of successful nuclear injection in our laboratory." Tian Bing came to help, purely watching the excitement.

Su Fan laughed awkwardly, and was interrupted by Gu Qiang's roar when he was about to say something.

"Inject!" Gu Qiang stood up abruptly, wildly like a rabbit that has completed the journey of life.

Everyone was very happy to watch, clapping and applauding for Gu Qiang's last five seconds of iron and blood injection.

Only Su Fan stared at the front blankly, as if something happened that he didn't want to recall.