Rebirth of the God-level Xueba

Su Xiankai and Fan Zhen were doing their own experiments, and their eyes swept across Yang Rui's table from time to time.

Since entering the "Ion Channel Laboratory", this is the first time they have seen Yang Ruizheng'er doing an experiment.

Although the person in charge of the laboratory does not have to have actual operational capabilities, just as an officer does not have to have a strong individual combat capability, but an officer with a strong individual combat capability is obviously more vulnerable to the soldiers. welcome.

Furthermore, Yang Rui is really younger, just like a young officer, always vulnerable to provocations by the soldiers.

Su Xiankai and Fan Zhenlong were still newcomers, and Yang Rui recruited them. They didn't dare to challenge Yang Rui's authority, but secretly observing them was fine.

Following their observation, both of them were obviously disappointed.

Yang Rui's operation level is too average.

Of course, this is a judgment made with high standards. Yang Rui has actually improved a lot from his previous experimental level. However, since the day when he became the head of the laboratory, everyone's expectations of him have also increased.

For example, Xu Zhengping is a middle-aged uncle. He still does not have his own laboratory. His experimental level has been trained for more than 20 years. Although many movements are incorrect when he learns. Not standard enough, but continuous experimentation for 20 to 30 years, non-standard movements also make Xu Zhengping's experiment operation fluent.

On the other hand, Yang Rui, even if certain movements are very standard, he still can't conceal the jerky under his hands.

"slow."

"Too slow."

This is the common idea of ​​Su Xiankai and Fan Zhenlong.

As for Xu Zhengping, he has long been accustomed to Yang Rui's not fast enough experimental moves. This time, what he didn't believe was Yang Rui's experimental plan.

What's a joke in doing DNA sequencing in a few days?

Gene sequencing technology began in 1977. At that time, there were methods for chemical degradation and determination of DNA sequence, as well as the dideoxy chain termination method currently done by Yang Rui. However, no matter which method it is, it is not easy to do DNA sequencing with this technology. , The error rate is very high and the efficiency is very low.

Of course, no matter how low the efficiency is, it is better than the small fragment sequence determination method invented in 65. The latter is like the potassium channel project made by Yang Rui. Not to mention it is all luck, the experiment is also extremely difficult.

Xu Zhengping believes that in those top laboratories, some researchers who are super familiar with DNA sequencing may indeed use a few days to complete DNA sequencing of plasmids. However, the ion channel laboratory is not a top laboratory, it is not even a provincial level. In the laboratory, Yang Rui is not a researcher who is super familiar with DNA sequencing...

In fact, there are currently no researchers in China who are super familiar with DNA sequencing.

Gene sequencing is indeed a very important biological experimental tool. If the "human gene sequencing" project is the peak of biology in the 20th century, gene sequencing can be regarded as the most important experimental tool in biological history.

However, throughout the 20th century, China has always been slow.

In 65 years, Sanger invented a small fragment sequence determination method and completed the determination of 120 nucleotides of E. coli 5sta. At the same time, the country was busy burning books and beating teachers, so naturally it was too late to learn.

In 1977, practical-level DNA sequencing methods appeared, and China was busy reprinting books to recruit teachers, and naturally it was too late to learn.

As an associate professor at Peking University, Xu Zhengping was exposed to dna sequencing just a few years ago. He did a complete experiment in total, which is considered to be the most important technology of modern molecular biology. Before his project involved genetic sequencing, he probably wouldn't do it a second time.

This is mostly the case for domestic researchers. In order to save resources, when graduate students learn these things, they also study theoretically.

Xu Zhengping used his cerebellum to think about it and knew that even if Yang Rui had done DNA sequencing, he did it once and a half.

You can see from his movements. It's a strange thing. With this technique, DNA sequencing can be done in a few days.

Xu Zhengping deeply believed that Yang Rui was in a loss, not to mention relying on this to get floating funds, these expensive reagents, etc., are considered to be in vain.

Yang Rui himself did not notice the sight of the people around him.

This experiment is indeed difficult for him and requires full attention.

The configuration of buffer and annealing mixture can be done by the assistant He Quangui and others. Even centrifugation and ethanol cleaning can also be done by the assistant, but when the solution is added and then electrophoresis is run, or heated and cooled on ice, It's all Yang Rui's work.

When Yang Rui was studying, the automatic sequencers used for DNA sequencing had reached the second generation. The automatic sequencers cannot be said to be fully automatic, but there are many manual steps that can be replaced.

Before the government suspends it, ordinary mothers can perform genetic sequencing and perform various prenatal screenings.

But in 1984, the current DNA sequencing technology did not even have the first generation. The United States may not have the first generation of DNA sequencers until next year. If the Chinese can afford it, it will take another two years—just Like an Apple mobile phone, you can afford to pay and still have to wait for the factory to produce it.

Without an automatic DNA sequencer, the manual is very complicated.

In the final analysis, DNA sequencing is to analyze the base sequence of DNA fragments. Adenine is a, thymine is t, cytosine is c, and guanine is g. These four letters circulate in various forms and constitute the basis of organisms. , And dna sequencing is to measure the order of these four bases.

Its principle is very simple, but it is not easy to do.

Because the base is invisible, to sequence, it must be reacted, and it is the correct response.

Accurately reacting the required bases, step by step, is the devil's footsteps for theology.

Yang Rui's footsteps were very slow, so slow that Xu Zhengping couldn't stand it, but a few hours later, after Yang Rui's electrophoresis ran, a clear gel appeared in front of several people.

"Weird pretty..." Fan Zhenlong was older and more socially motivated. He wanted to shoot Yang Rui a flattery, and finally found a chance. Although the shoot was relatively light, he was finally a flattery, and he also gained Yang Rui's. Smile.

Su Xiankai's reaction was a little slower, and he immediately said, "It's pretty..."

No matter how young Yang Rui was, when he was the head of the laboratory, the lifeblood of everyone in the laboratory was in Yang Rui's hands. From funding to publishing papers, everyone must be restrained by Yang Rui. If you meet a better laboratory manager, you can have more funds, fewer boring tasks, and less trouble if you publish your papers. When encountering a laboratory leader with poor moral character, not only does the funding cost less, but the results are more demanding. What's more, some laboratory leaders not only need to be the corresponding author, or even the first author, but they dare to take the author’s Signing someone outside the laboratory, doing favors or even pure transactions.

When Su Xiankai and Fan Zhenlong arrived for the first time, they couldn't figure out Yang Rui's temper, but at least they knew that it was always right to follow Mao.

Yang Rui recorded the results and continued to repeat the process just now.

This time, for a few more hours, I still got a beautiful gel and got another round of praise.

Yang Rui repeated it again, again and again.

From morning to night, Yang Rui didn't stop except to eat. In the afternoon, he even added a set of experiments.

Su Xiankai and others have nothing to praise, but Xu Zhengping's eyes are straight.

Actually get the accurate result directly every time!

In the age of manual experimentation, this is very difficult.

After all, this is also a new DNA sequence that is fresh and **. If it is a repeated experiment, it is of course much simpler to follow the answers of others, just like the repeated DNA sequencing done by Fan Zhenlong and Su Xiankai, but the new DNA sequencing will always give some undesired answers.

It's just the last step to run electrophoresis, and you often encounter situations where you can't get it out, and then you fall short.

Yang Rui was slow in every step, but he did not encounter any mistakes, which made Xu Zhengping almost dare not think about it.

But the scene that he didn't dare to think about now appeared in front of him.

This is like a final exam. Only one student has scored 100 points. If you say that he is plagiarism, this is not right. If it is a real exam, there are still correct answers. Look, Yang Rui is doing it now, but the whole world The unique s5 fragment of Tengchong thermophilic bacteria was sequenced. Not to mention that foreign researchers did not have Tengchong thermophilic bacteria, that is, they did not sequence and published public papers. Xu Zhengping, who often checks the information, is very convinced.

"It's so beautiful." At eight o'clock in the evening, Xu Zhengping gave a sincere compliment as he watched Yang Rui make another clear version of the gel.

Yang Rui smiled, and said, "It's just an oil seller."

"You are a groundbreaking experiment. It's useless to be familiar with it." Xu Zhengping didn't believe him.

Yang Rui actually said that he missed his mouth. He used the answers in his mind to reverse the sequence. Of course, it was accurate every time. Naturally, he would not refute at this time. He pretended to be humble and smiled again, and said: "Do one more thing. Team, I’ll take a rest, if you want to go back, go back first."

"Don't worry, let's take a look and learn." Fan Zhenlong made another flattery.

"You all have a family, don't go back to accompany your wife, and see what is the meaning of my experiment."

"Spiritual support." Fan Zhenlong replied short and powerfully.

"I'll give you a hand. My experiment is finished, and all the reagents prepared today are used up." Su Xiankai was more direct.

Many reagents, such as buffers, are ready to be used. Generally speaking, they are configured when they come in the morning, and how many are configured according to the progress. When used up, the experiment should be ended. Of course, some demanding experiments can't even be stored for a few hours, so they can only be reconfigured occasionally.

Su Xiankai had already prepared more reagents, but didn't plan to make it to 8 o'clock in the evening. Now he wants to continue his experiment, but he has nothing to do.

Yang Rui gave him a position and said, "Then stay with me for another two hours. I will finish the sequencing these days. It would be good to get tens of thousands of dollars more, right?"

"Tens of thousands of yuan is of course good, but after a few days, it is really choking." Su Xiankai told the truth~www.wuxiaspot.com~Yang Rui smiled and said: "I'm tired, but I should be able to finish it."

Su Xiankai couldn't help saying: "The s5 fragment of Tengchong Thermophile has a total of 126 base pairs."

126 base pairs are relatively short. Yang Rui made 5 in a day, which was also extremely fast, but dividing by elementary school mathematics, he knew that this project would not be completed in a few days.

In fact, this has taken off quickly. In foreign countries, many laboratories have sequenced a gene chain and tested it for one or two years. The various strange problems encountered during the period are exhausted.

It's already amazing that Yang Rui did so smoothly today, but if it's smoother and faster, Su Xiankai really can't shoot such an excessive flattery.

Yang Rui didn't have to explain, but just continued to do it.

No one noticed, but his current proficiency has increased.

……

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