I reach the pinnacle of life by learning

Qiao Yu was silent for a moment: "No."

Shi Wen lowered his head, stretched out his hand and made a "please" gesture: "Then you can go."

Qiao Yu coughed: "Professor Shi, actually, I am here this time because I have an experimental project related to structural biology. I hope you can help me."

Shi Wen immediately crossed his arms and said, "Why don't you ask Ye Qinxue?"

If at this time, Shi Wen is in front of a sycophant, the answer is naturally "Professor Ye is inferior to you in terms of structural biology", so that Professor Shi can also brag to others at the wine table in the future.

But even though Qiao Yu has been away from the workplace for a long time, she still understands the essence of office struggle.

He replied: "Professor Ye is quite busy recently, let me come and find you."

Shi Wen was barely satisfied with this answer.

"Okay, show me what you have."

Qiao Yu handed over the folder he carried with him.

Shi Wen weighed it, and said with a smile: "It's heavy, you've done a lot of homework, let me see how you're doing."

He turned to the first page, glanced at the title, and muttered to himself: "Cryo-electron microscopy single particle analysis technology..."

As a kind of structural analysis method, cryo-electron microscopy single particle analysis technology is one of the hot research fields in recent years.

At present, this technology has been able to resolve proteins with a molecular weight of more than 3000kDa and stable biochemical properties to a level close to atomic resolution.

However, if we want to go a step further and carry out high-resolution analysis of small molecular weight proteins, there is no doubt that there is more than enough power.

Because under the existing technology, it is difficult to achieve proper contrast for small molecular weight protein particles in frozen samples.

The project topic given by Qiao Yu is to measure the three-dimensional reconstruction of streptavidin protein under the cryo-electron microscope*.

"How do you think of measuring streptavidin protein?" Shi Wen asked.

Qiao Yu answered very directly: "The size is suitable, and it is easy to extract."

Streptavidin is a kind of small molecular protein, only 66 kilodaltons.

Don't look at the series of papers published in journals, in fact, in recent years, many scholars have published papers around the word "minimum".

Today Professor X published a high-resolution three-dimensional map of a 300kDa protein, and tomorrow Professor Y will publish a 200kDa protein analysis map.

One article for your water, one article for my water. At the end of the water, it was found that there was no way to measure it down, so everyone researched in another direction.

Anyway, the cryo-electron microscope is there, if you make more troubles, the result will always come out.

Currently, the "smallest" record holder is Professor Takeuchi of the University of Tokyo.

He mapped a secreted protein with a size of about 100kda. The paper was published in "Molecular Structure" two months ago, which is a large journal with an impact factor of 12.

If Qiao Yu wanted to do this kind of research, he could only do it in smaller proteins.

As soon as the experts make a move, they will know if there is any.

Shi Wen didn't expect that Qiao Yu wrote so many words, but he really didn't count the words, but had stock in his stomach.

He saw the end from beginning to end, and found that the opponent was well prepared.The experimental method given is indeed feasible.

He pondered for a moment, then folded the papers and put them on the table.

Professor Shi doesn't like to save face for others, being his graduate student is definitely hell-level difficulty.

Over the years, Shi Wen has thrown countless papers into the trash can after reading them.

And Qiao Yu's documents can still be left on the table, which is enough to see his affirmation.

Shi Wen: "Yes, where is your problem?"

"I was wondering how you improved the contrast of protein particles in your photographs when doing structure elucidation,"

Qiao Yu frowned slightly: "I tried reducing the accelerating voltage, adding physical gratings and staining. But when the protein size is below 200 kilodaltons, these methods obviously lose their effect."

It is not only the cryo-electron microscope that needs voltage, but also the transmission electron microscope.

Shi Wen didn't care for a while, and replied: "You buy a spherical aberration corrector and a voltage phase plate, calibrate the data yourself, and then replace the carbon tube with a single-layer large single-crystal graphene grid. It should be fine. .”

This is the benefit of having a big cow mentor.

Often the point you have been struggling with for a long time is the detour he has gone through, so that you don't take it as a secret at all, and point out the key point casually.

And a mentor who has no experience in this, such as Zhang Kaiwei, probably can only scratch his head with Qiao Yu face to face, thinking about what the problem is.

Qiao Yu said sincerely: "Thank you, Professor. When the paper is published, I will definitely put your name on it."

Shi Wen waved his hand: "I don't care about your work? I'm not the kind of water injection professor, so there's no need to engage in formalism with me."

After finishing speaking, he said, "Do you need a cryo-electron microscope for your experimental structure diagram? We happen to have one at Tsinghua University, and another one is being airlifted. It's not good to borrow it out, so you can come directly when the time comes. I'll open the access control card for you. "

Qiao Yu bowed slightly: "Don't bother you."

"It's okay, our school is so close, it doesn't matter if you come to visit more often." Shi Wen suddenly let out a hearty laugh, and clapped his big hands on his legs very loudly, "You can use the cryo-electron microscope casually when I'm in the laboratory, it's not a big problem. "

If this remark was heard by the scientific research dog under Professor Shi, they might burst into tears of grievance in an instant.

Cryo-electron microscopes are expensive, and it cost more than 2000 million yuan to buy one, which is really a treasure.Everyone in the Academy of Life Sciences wants to touch it, but Professor Shi is guarding it like a thief.

Now, they use cryo-electron microscopes to write applications and then lottery.

Qiao Yu had no choice but to say, "Thank you, Professor, but I really don't need it. I have it in my laboratory."

"Don't be my guest..." The smile on Shi Wen's face suddenly froze, and his eyes widened suddenly, "What, did you Yanda also buy it?!"

Why hasn't he heard of such a big event?

Because Tsinghua University is good at science and engineering, among so many universities in China, it receives the most financial allocations every year.

In Tsinghua University, the universities that receive the most scientific research funds are the universities under the Ministry of Science and Technology, including physics, chemistry, and biology.

Even so, applying for a cryo-electron microscope would require the whole Tsingda University Academy of Biological Sciences to tighten their belts for a whole year.

If Yanda bought it, I am afraid that Ye Qinxue will show off in front of him when the application is passed, how can he hold it so that there is no sound at all?

Qiao Yu took out the excuse he had prepared long ago: "I am employed by Xun'an Biopharmaceutical Company, and this is bought by the company."

The problems on the books will be settled by the system itself.

Professor Shi looked up to the sky angrily and shouted: "Don't think I don't know, isn't this your family business?"

Xunan Biopharmaceuticals is not listed. In the shareholding structure, a foreign company called HKB accounts for 90.00%. This company is registered in the Cayman Islands, a world-renowned tax haven; and the other [-]% is in the hands of Ms. Qiao Yue.

According to the data, although Ms. Qiao Yue only holds [-]% of the shares, she is the ultimate beneficiary of the entire company.

Thanks to the previous awards ceremony of the Clay Institute in the United States and the media's spare no efforts in publicity, the whole world now knows that Qiao Yue opened Xun'an Biopharmaceuticals.

Qiao Yu: "... Rumors. They are all rumors."

What kind of family business is this? It is obviously his personal business, the leather bag company, which is used to patent and finance himself.

Shi Wen didn't want to listen to his explanation, so he pointed to the door: "Let's go if you have nothing to do, I still have to go to work."

It was almost ten o'clock when Shi Wen came out after finishing his official duties.

He rushed to the parking lot with his briefcase under his arm, and unexpectedly ran into Zhou Shenxing who happened to be coming home from get off work.

Shi Wen has a weird temper and is famous in the whole school.As a result, this time he took the initiative to say hello to Zhou Shenxing, which surprised him very much.

"Qiao Yu from Yan University came to see me today." After saying hello, Shi Wen went straight to the point.

Zhou Shenxing was very surprised: "What's going on?"

"There is something wrong with his research, come here and ask."

Zhou Shenxing: "Oh, so..."

If it is a mathematical problem, Dean Zhou can still discuss one or two, but in other fields, it is purely powerless.

"It's no wonder your Mathematics College keeps poaching the corners of Yan University," Shi Wen sighed in admiration, "I would say that most graduate students in China are not as good as technical schools. At least their technical schools can meet the operating standards. The format of the thesis is wrong. It’s not easy to meet someone with full skill points, so don’t try hard to poach people.”

The more Shi Wen thought about it, the more angry he became: "Damn it, why did Yan University take advantage of it?"

The more Zhou Shenxing thought about it, the more angry he became: "That's right. Wu Zhitian, a sophomore, has been showing off in front of me every day recently!"

The two principals, who were not very familiar with each other, looked at each other from a distance because the hero saw the same thing, and suddenly felt that it was rather late to meet each other.

After getting the answer from Professor Shi, Qiao Yu immediately got into work.

Both the spherical aberration corrector and the voltage phase plate can be borrowed from Yanda, following the path of Zhang Kaiwei.

Zhang Kaiwei took the equipment and went to the No. 02 laboratory of Xun'an Biopharmaceutical for the first time.

Not to mention the location and the decoration, just the thing in the corner that looks like a cryo-electron microscope makes Zhang Kaiwei a little bit from ear to ear.

Adhering to the principle of making the best use of everything, Qiao Yu asked Professor Zhang, who came to deliver things, to help out by the way.

Instead of being dissatisfied, Zhang Kaiwei was very excited.

Previously, in his genetic laboratory, Zhang Kaiwei's daily work was repetitive and boring.Finally, there is a new experiment to do, and his eyes are shining.

Not to mention, the legendary cryo-electron microscope can also be used in this laboratory!

When Qiao Yu was still using SDS buffer to concentrate small molecule peptides, Zhang Kaiwei had found a random protein and put it under a cryo-electron microscope, tears filled his eyes as he looked at the macromolecule analysis picture on the screen.

This is fucking scientific research!

What he was doing before, the technical difficulty is not as good as the postpartum care of sows.

At the end of the day, Professor Zhang asked coyly, "Can I come back tomorrow?"

Qiao Yu was quite surprised: "Of course, welcome anytime."

He also thought that Zhang Kaiwei would be impatient with these trivial experiments.

Professor Zhang was as happy as a child who bought a new toy.

Qiao Yu looked at his back, and said to the system thoughtfully: "Although there are many scoundrels in the academic world, I think most of the scholars I have met are actually very good."

From Lin Feng to Zhang Kaiwei, from home to abroad, Qiao Yu received far more kindness than malice.

Most of the people acted like seniors, shielding him from the wind and rain.

The system wanted to say that it was because you were worth it, but when it came to the mouth, it felt too hypocritical.

So it replied: "Indeed."

The author has something to say: I wanted to write more, at least until Qiao Qiao finished publishing the paper, but I am too sleepy 555, I will add more tomorrow.

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* There is a reference paper: "Single particle cryo-EM reconstruction of 52kDastreptavidinat3.2Angstromresolution of Streptavidin protein with a molecular weight of 52kDa at 3.2 Angstrom resolution".