This doctor has a system

Chapter 92 92. Cough variant asthma 2
Gao Feng has headaches all the time, and he is most afraid of talking about his illness to the elderly. After talking about it, he will find that the two people are not on the same channel at all.

"It's not an allergy department, it's an allergy department." Gao Feng patiently explained to the old man, "Asthma is an allergic disease, so the triage nurses do it."

"It's not the pervert we're talking about!"

"This cough variant asthma is a type of bronchial asthma that doesn't have the expiratory dyspnea typical of asthma."

"The main symptom of this disease is cough, but if it is not treated in time, it may turn into typical asthma."

The patient's condition does not need to be hospitalized for the time being, just go back and inhale the medicine, "Old lady, if you are worried, you can go to the town hospital near your home for nebulization treatment."

The old lady thought about it, "I've come here, if not, I'll stay in your hospital for a few days."

"The consumption here is a bit high." Dong Qianqian came up, "The bed fee and nursing fee alone cost 160 yuan a day, which cannot be reimbursed."

"So expensive?! How much is the medicine that day?"

"The medicine is estimated to cost 50 yuan."

"What?!" The old lady was dumbfounded, the bed fee was more expensive than the drug fee, "Forget it, we still can't live anymore, let's go back to the town hospital." The old lady made a decision.

"Take the lung function report and let the doctor over there look at it." Gao Feng explained to her, "People will understand what's going on after seeing it."

After sending the old man away, Gao Feng breathed a sigh of relief. This old lady is still one of those who are easy to communicate.

After get off work in the afternoon, Gao Feng went to the scientific research building to check on the mice, where Qi Nannan was feeding the mice.

"One died." She saw Gao Fenghui's report.

That's okay, as long as you don't die too many at once, it means that the experiment is relatively smooth.Of course it's only been a day, so it's a bit early to say.

In the following time, Gao Feng and Li Youliang made preparations for cell fusion and selection of hybridoma cells.

These two tasks have high requirements for personal operation. For cell fusion, it is necessary to mix spleen cells and 1×10^7 myeloma cell SP2/0 in a 50 ml fusion tube, and then add incomplete culture Base to 30 ml, mix well.

Then use a centrifuge to centrifuge at 1000 rpm for 5-10 minutes, and suck up the supernatant as much as possible.

Gently tap the bottom of the fusion tube on the palm of your hand to loosen the precipitated cells evenly;

Use a 1 ml pipette to add 30 ml of preheated 50% PEG within 1 seconds, and stir gently while adding.

The suction pipette was allowed to stand for 1 min.

Add preheated incomplete culture medium to stop the PEG action, add 2 ml, 1 ml, 2 ml, 3 ml, 4 ml, 5 ml every 10 min.
800rpm, 5 minutes; discard the supernatant.

Add 5 ml of complete medium, gently blow and aspirate the pelleted cells to suspend and mix well, then add complete medium to 40-50 ml.Aliquot 96-well cell culture plates, 100ul per well, and then place the culture plates in a 37°C, 5% CO2 incubator for culture.

After 6 h, selection medium was added.50ul per hole. After 3 days, replace the liquid with half of the selection medium.

Always observe the growth of hybridoma cells, and when they grow to more than 1/10 of the area of ​​the bottom of the well, aspirate the supernatant for antibody detection.

These steps feel troublesome to write out, let alone do it.Gao Feng and Li Youliang practiced patiently until late every day, but they always felt that the efficiency was very low.

"Let's call Jia Ran and Cheng Song over." Gao Feng probably had the final say in his mind. According to this situation, the two of them would be exhausted and couldn't do it.

"There are still too few people!" Li Youliang said to Gao Feng, "We will see if we can recruit a few more people in the future."

"Let's talk about it after the results are produced. I guess it's not appropriate to call anyone now." Gao Feng also thought about this, but he felt that there was no rush. At present, the whole team is generating electricity for love. Easy to manage.

In the scientific research experiment, we are very afraid of the situation of picking things up. Generally, at least two people should be responsible for the key links, so as to avoid the interruption of the experimental process due to unexpected circumstances.

Gao Feng now requires himself to be familiar with each step, also to prevent this situation.

Hu Jiaran came very quickly, he was full of enthusiasm for the new task, Gao Feng taught him twice, and he quickly mastered the tricks.

"Jiaran, you can go to graduate school." Li Youliang couldn't help but said, "Your hands-on ability is too strong."

"I'm not good at English, and I didn't pass the mark last year." Hu Jiaran chatted with Li Youliang while doing the work, "I got 31 points in English for the postgraduate entrance examination, and I didn't pass level 6 when I graduated. I was dizzy when I saw English. sharp."

"You." Li Youliang shook his head, "That's really helpless."

"By the way, there is one more thing." Li Youliang said to Gao Feng, "Now everyone is going back late at night because of the experiment, which is very inconvenient."

"My teacher has a vacant house at the intersection of Jinger Road and Hongzhuan Road. It's a three-bedroom house. No one has lived in it for several years."

"Everyone can live there, just bring a quilt, and everything else."

"Okay, I'll talk to Cheng Song and Qi Nannan later." Hu Jiaran replied.

In fact, Gao Feng also considered this issue, and planned to rent a house nearby. Unexpectedly, Li Youliang worried more than him.

"Is it suitable?" Gao Feng looked at Li Youliang, "Have you told Director Zhao?"

"I have the final say on the house now." Li Youliang was a little proud, "My teacher said he would sell the house to me at the price when I bought it."

"The teacher asked me to live there first, and wait until I have money."

The house was bought by Director Zhao when the market opened, with an average price of 4500 per square meter, and now it is almost 14500 per square meter, an increase of nearly 1 per square meter.

"I'll go!" Gao Feng exclaimed.

"Director Zhao is too kind to you." Hu Jiaran was also surprised when he learned about the situation of the house.

"Don't say it out there." Li Youliang explained to the two of them, "Otherwise, my senior sisters and senior brothers should bother my teacher."

Director Zhao is very kind to the students she leads. She only recruits one postgraduate student every year. In recent years, the students have been arranged for jobs after graduation.

Every year on Teacher's Day, a large group of disciples come to the hospital to look for her, "You people pay me 2000 yuan every year." Director Zhao laughed and scolded at the dinner table.

Li Youliang's family's financial conditions are not good. Director Zhao has heard about it for a long time. The student has performed really well in the past three years. She went back to discuss the house with her lover.

The lover immediately agreed, "Youliang is a really nice guy, and it's not easy to stand on the back of a horse. Help if you can, and we're not bad at that."

Director Zhao's lover runs a law firm and is well-known in HN province. He spends his money in sacks every day, and he doesn't like anyone with three melons and two dates.

"Then you are short of a wife right now." Gao Feng said, "I'll let Xinyao find one for you."

"Okay, preferably a university teacher or civil servant or something."

"Can't doctors and nurses do it?" Gao Feng stopped what he was doing, and after more than an hour, his wrists were sore.

"I don't want it anymore. Both of us are too busy, who cares about the family. My parents are not in good health, and they will be tired after taking care of the children." Li Youliang thought a lot.

"It's better to find someone with a relatively leisurely job."

　The past two days have been a bit busy, and the update time is a bit irregular.

　　
　
(End of this chapter)