This doctor has a system

Chapter 93.

"You always think so much." Gao Feng pondered for a while, "It's actually not very good."

"It's mainly because of family economics. I have been like this since I was a child, and I like to calculate." Li Youliang laughed at himself.

"Brother Youliang, you are considered to be an outstanding person now, and your social status has risen to a higher level." Hu Jiaran complimented him.

"How can I get ahead? The main reason is that the starting point is too low."

Several people chatted for a while, and then began to repeat the previous experimental steps.

Li Youliang left for a while earlier. Director Zhao will go to the outpatient clinic tomorrow, and he will be on duty. He has been busy with Xiong Huaxin recently, and the junior sister is a bit overwhelmed by herself.

"Jiaran, do you have any plans for the future?" Gao Feng asked.

Hu Jiaran thought for a while, "I don't know, my dad told me to go back to work in the county hospital after regular training."

"I'd like to stay here, but it's not easy to get a job. I can't get into a better hospital. It's even worse than our county hospital."

"My father is the deputy director of the county hospital, and my mother works at the county women's federation, and someone takes care of her in her hometown."

"Your starting point is already higher than 90% of the people." Gao Feng said with a smile, "Do you have other brothers and sisters?"

"There is an elder sister who is married and also works as a civil servant in our county." Hu Jiaran smiled when he mentioned this elder sister, "He is very kind to me, and she came here to see me a few days ago."

"Yeah, it's good to have an older sister. She's much better than a younger sister. My younger sister will only find you a lot of things." Gao Feng expressed his feelings.

When Gao Zhenzhen was in elementary school, she fought with others and was beaten by others. She cried and went to the class to find him.

The other party also asked his elder brother to come over, he was very big, "Well... It's normal for their children to have conflicts, I think we should still value peace."

However, the other party's education level was too low (elementary school education), and he didn't talk nonsense with him at all, so Gao Feng was beaten up solidly.

"You won't have any brothers anymore." Gao Feng said to Gao Zhenzhen on the way home.

"Jiaran, if I make achievements in the future, you can do it with me." Gao Feng threw an olive branch to Hu Jiaran, "I think you are very capable."

"Okay." Hu Jiaran agreed happily.
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Two weeks passed quickly, during this period a total of 2 mice died, 21 of which died within 16 days after the antigen injection, and the condition of the latter mice was good.

Gao Feng was very satisfied, and specially praised Qi Nannan, "Look, I say you are the most careful."

There was only a slit left in the latter's smiling eyes.

Everyone arrived at night, and everyone began to draw blood from the mice to test the titer, to see how the immune effect was, and Gao Feng was very excited by the results.

"Let the manufacturer send 6 more 10-200-week-old mice." Gao Feng explained to Li Youliang, "It's time to prepare macrophages."

The next day happened to be Saturday, except for Cheng Song who was going to the clinic with the teacher, no one else went to the department in the afternoon.

Qi Nannan was in charge of killing all the newly acquired mice, "I'll help you." Hu Jiaran wanted to put on gloves.

"No, that's all. I'm not enough to kill myself." Qi Nannan rattled, rattled again, with a smile on his face.

Hu Jiaran shivered suddenly, "Do you think she is a pervert?" He said to Li Youliang, "The rat killer!"

"It's different from other girls." Li Youliang glanced at Qi Nannan.

Cut the mouse skin with sterile scissors to expose the peritoneum.Inject 6 ml of culture solution with a straw, rinse repeatedly, suck out the rinse solution, and put it into a 10 ml centrifuge tube.

Suspend with 20% calf serum culture medium, adjust the cell number to 1*105/ml, add 96ul/well to 100-well plate.Put into 37 degree incubator culture.

In this way, the preparation of macrophages is completed, and the next step is the preparation of myeloma cells.

48-36 hours before fusion, the myeloma cells were expanded and cultured. On the day of fusion, gently blow off the cells from the bottle wall with an elbow dropper and collect them in a 50 ml centrifuge tube or a fusion tube.

Then centrifuge at 1000 rpm for 5-10 minutes with a centrifuge, and discard the supernatant.

Add 30 ml of incomplete medium, centrifuge and wash once.Then resuspend the cells in 10 ml of incomplete medium and mix well.

myeloma cell suspension
This step looks simple, but the operation is very fragmented, and several people are tired and backache.

The next step is the preparation of spleen cells,

Take the immunized mice, remove the eyeballs to collect blood, and separate the serum as the positive control serum for antibody detection.

At the same time, the mice were killed by cervical dislocation, soaked in 75% alcohol for 5 minutes, fixed on a petri dish, and the skin on the left side of the abdomen was lifted. The spleen can be seen, and the ophthalmic scissors were replaced. For the peritoneum, take out the spleen and place it in a plate filled with 10 ml of incomplete medium, wash it gently, and carefully peel off the surrounding connective tissue.

This step was completely Hu Jiaran's performance time. Gao Feng had to concentrate to keep up with his steps. Li Youliang and Cheng Song's performance was even worse. They finished 2 and found that Hu Jiaran had already done 8.

"Why don't we leave it all to Jiaran." Cheng Song was in a bad mood, "It's too difficult for me."

"Don't compare yourself to Jia Ran." Li Youliang comforted him, "This is our normal operation speed, he is a special case, it may be the proficiency of both hands that he has practiced for a long time alone."

Due to cell fusion and selection of hybridoma cells, they couldn't stay for too long in the middle, and several people were busy until 3 o'clock in the morning.

"Okay, the next step is the screening of monoclonal antibodies. You can take a break." Everyone dragged their tired bodies and left the scientific research building.

Gao Feng slept until noon the next day, Li Xinyao pulled him up, "Let's go to my house for dinner."

"My aunt and the others are here too, I don't know why they are here." Li Xinyao was a little unhappy, it seemed that she still had a bad impression of her aunt.

"Oh, Gao Feng is back." Li Lingyu quickly stood up from the sofa, "Sit down and rest."

Husband Mao Jianfeng hurried to get the disposable paper cup on the table to fetch water for Gao Feng.

Li's father and Li's mother
"Bewitched." Li's mother looked at Li's father.

Father Li was also a little puzzled, but his expression gradually became serious. His sister and brother-in-law are people who have no money and can't afford to be early. He must be focusing on Gao Feng.

"Are you tired? I heard from Xinyao that you have been busy with work recently, so you have to pay attention to your health." Li Lingyu said with concern, "You are all doctors, and we understand the importance of good health better than we do."

Gao Feng said a few words along with him. After all, he is an elder, and his attitude this time is also very good.

"Didn't Jianfeng build two more gas stations recently, and I'm thinking of sharing one for you." Li Lingyu said to Li's father, "It's a lot of money in a year, anyway, it's much better than you going to work."

Father Li's expression became even worse after hearing this, "Little sister, come down here, I have something to ask you."

He turned around and took Li Lingyu to the guest bedroom, "What are you doing? Tell me honestly!"

(End of this chapter)